The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA.
These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus.
The protein encoded by MED14 (mediator complex subunit 14) is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1.
This protein is also a component of other multisubunit complexes e.g.
thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
This protein contains a bipartite nuclear localization signal.
MED14 is known to escape chromosome X-inactivation.
Applications:Suitable for use in Immunohistochemistry, Immunofluorescence and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human CRSP150. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa670-750 from the Internal region of human CRSP150.
