MAP3K8 is an oncogene that encodes a member of the serine/threonine protein kinase family.
The mitogen-activated protein kinase kinase kinase 8 localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways.
This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB.
This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation.
MAP3K8 may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminal.
The shorter isoform has been shown to display weaker transforming activity.
Alternate splicing results in multiple transcript variants that encode the same protein.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Cot. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human Cot around the non-phosphorylation site of S400.
