84-5442-52　［取扱停止］Cortactin 30ul　534996

特徴

• This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck.
• The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts.
• This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells.
• During apoptosis, the encoded protein is degraded in a caspase-dependent manner.
• The aberrant regulation of this gene contributes to tumor cell invasion and metastasis.
• Three splice variants that encode different isoforms have been identified for this gene.
• [provided by RefSeq, May 2010]CTTN (Cortactin) is a Protein Coding gene.
• Diseases associated with CTTN include ampulla of vater adenocarcinoma and ethmoid sinus cancer.
• Among its related pathways are Signaling by GPCR and Signaling by Rho GTPases.
• GO annotations related to this gene include profilin binding.
• An important paralog of this gene is DBN1.
• ontributes to the organization of the actin cytoskeleton and cell shape (PubMed: 21296879).
• Plays a role in the formation of lamellipodia and in cell migration.
• Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity).
• Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (By similarity).
• Plays a role in the invasiveness of cancer cells, and the formation of metastases (PubMed: 16636290).
• Plays a role in focal adhesion assembly and turnover (By similarity).
• In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (PubMed: 20861316).
• Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed: 17959782).
• Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity).
• Required for stabilization of KCNH1 channels at the cell membrane (PubMed: 23144454).
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.