COL4A1 (collagen type IV alpha 1 chain) encodes a type IV collagen alpha protein.
Type IV collagen proteins are integral components of basement membranes.
COL4A1 shares a bidirectional promoter with a paralogous gene on the opposite strand.
The protein consists of an N-terminal 7S domain, a triple-helix forming collagenous domain, and a C-terminal non-collagenous domain.
It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins.
In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties.
Mutations in COL4A1 cause porencephaly, cerebrovascular disease, and renal and muscular defects.
Alternative splicing results in multiple transcript variants.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:10,000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Collagen IV. Species Crossreactivity: human, mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa1620-1670 from Collagen IV.
