COL5A3 encodes an alpha chain for one of the low abundance fibrillar collagens.
Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains.
Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen.
This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations.
Mutations in COL5A3 are thought to be responsible for the symptoms of a subset of patients with Ehlers-Danlos syndrome type III.
Messages of several sizes can be detected in northern blots but sequence information cannot confirm the identity of the shorter messages.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human COL5A3.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa190-270 of human COL5A3 at N-terminal.
