Type IV collagen proteins are integral components of basement membranes.
This gene shares a bidirectional promoter with a paralogous gene on the opposite strand.
The protein consists of an N-terminal 7S domain, a triple-helix forming collagenous domain, and a C-terminal non-collagenous domain.
It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins.
In addition, proteolytic cleavage of the non-collagenous C-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties.
Mutations in COL4A1 cause porencephaly, cerebrovascular disease, and renal and muscular defects.
Alternative splicing results in multiple transcript variants.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human COL4A1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa30-110 of human COL4A1 at N-terminal.
