Cofilin is ubiquitously expressed in eukaryotic cells where it binds to actin, thereby regulating the rapid cycling of actin assembly and disassembly essential for cellular viability.
Cofilin is a low molecular weight protein that binds to filamentous (F) actin by bridging two longitudinally associated actin subunits changing the F-actin filament twist.
This process is allowed by the dephosphorylation of cofilin ser-3 by factors such as opsonized zymosan.
Lim kinase 1, a serine kinase, phosphorylates cofilin and renders it unable to bind and depolymerise F-actin.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Cofilin (phospho Ser3). Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human Cofilin around the phosphorylation site of S3.
