Chloride channels (CLCs) regulate cellular traffic of chloride ions, a critical component of all living cells.
CLCs are involved in membrane potential stabilization, signal transduction, cell volume regulation and organic solute transport.
CLCC1 (Chloride channel CLIC-like protein 1), also known as MCLC (Mid-1-related chloride channel) or KIAA0761, is a 551aa multi-pass membrane protein that belongs to the chloride channel MCLC family.
CLCC1 is related to the Saccharomyces cerevisiae protein Mid-1 and is believed to function as an intracellular chloride channel that is expressed in lung, brain, muscle, liver and testis.
Localizing to intracellular compartments such as the Golgi apparatus, the endoplasmic reticulum (ER) and the nuclear envelope, CLCC1 is expressed as four isoforms due to alternative splicing events, namely hMCLC-1, hMCLC-2, hMCLC-3 and hMCLC-4.
Applications:Suitable for use in Western Blot, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:200-1:1000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human CLCC1.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa360-440 of human CLCC1.
