The protein encoded by CISH contains a SH2 domain and a SOCS box domain.
The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family.
CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling.
The expression of CISH can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells.
Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor.
Multiple transcript variants encoding different isoforms have been found for CISH.
CISH (cytokine inducible SH2 containing protein) is a protein coding gene.
Diseases associated with CISH include bacteremia and malaria.
Among its related pathways are Jak-STAT signaling pathway (KEGG) and IL-2 signaling pathway.
Applications:Suitable for use in Immunohistochemistry (paraffin), ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:50-1:300ELISA: 1:10,000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human CIS. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa30-90 from human CIS.
