RRP8 (ribosomal RNA processing 8, methyltransferase, homolog (yeast) is a protein coding gene.
Diseases associated with RRP8 include bowen-conradi syndrome and lymphatic system cancer.
Among its related pathways are gene expression and activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3.
GO annotations related to this gene include poly(A) RNA binding and methylated histone binding essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes.
The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at Lys-9 (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus.
In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase.
Its substrates are however unknown.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Cerebral 1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa240-320 of human Cerebral 1.
