CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain.
It collaborates with CEP110, being involved in the suppression of a cilia assembly program.
CEP97 is required for correct spindle formation and has a role in cytokinesis.
It is required for recruitment of CEP110 to the centrosome.
This protein has a calculated MW ~97kD, although the protein migrates at a molecular weight of approximate 140kD.
The protein is highly acidic, with a pI ~4 providing a potential explanation for its reduced mobility in SDS-PAGE.
[PMID: 17719545].
This is a rabbit polyclonal antibody raised against the C-terminal of human CEP97.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Cep97. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa550-630 from human Cep97.
