Cytokinesis occurs during the late stages of mitosis and describes the process by which the cytoplasm of one cell is divided to create two subsequent daughter cells.
Centriolin, also known as CEP110, is a coiled-coil centrosomal protein that is required for centrosome maturation and correct centrosome function.
During abscission, the last step in cytokinesis, Centriolin both coordinates and grounds vesicle-fusion and vesicle-exocyst complexes to the midbody of the daughter cells.
Acting as an anchor for the protein complexes needed to complete separation of the two cells, Centriolin initiates and controls vesicle-mediated cell cleavage.
Through its ability to signal initiation of the last step of vertebrate cytokinesis, Centriolin regulates cell proliferation and contributes to proper entry into the S phase of mitosis.
Applications:Suitable for use in Immunohistochemistry, ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Centriolin.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the C-terminal region of human Centriolin.
