Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes.
CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere.
Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
The protein is a replication-independent histone that is a member of the histone H3 family.
Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Applications:Suitable for use in Immunofluorescence and ELISA.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:200-1:1000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human CENP-A (phospho Ser7).
Isotype：IgG
Calc Applications Abbrev：E IF
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human CENP-A around the phosphorylation site of S7.
