CDK12 (cyclin dependent kinase 12) is a protein coding gene.
Among its related pathways are DNA damage and formation of HIV elongation complex in the absence of HIV tat.
Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation.
Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability.
Preferentially phosphorylates Ser-5 in CTD repeats that are already phosphorylated at Ser-7, but can also phosphorylate Ser-2.
Required for RNA splicing, possibly by phosphorylating SRSF1/SF2.
Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human CDK12. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa50-130 from human CDK12.
