CDK2 encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation.
Cyclin dependent kinase 2 is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle.
Activity of this protein is especially critical during the G1 to S phase transition.
This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B).
Alternative splicing results in multiple transcript variants.
Applications:Suitable for use in Western Blot, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:200-1:1000ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Cdk1/2/3 (phospho Thr14). Species Crossreactivity: mouse, rat, monkey
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide derived from human Cdk1/2/3 around the phosphorylation site of T14.
