CDCA4 (cell division cycle associated 4) encodes a protein that belongs to the E2F family of transcription factors.
This protein regulates E2F-dependent transcriptional activation and cell proliferation, mainly through the E2F/retinoblastoma protein pathway.
It also functions in the regulation of JUN oncogene expression.
This protein shows distinctive nuclear-mitotic apparatus distribution, it is involved in spindle organization from prometaphase, and may also play a role as a midzone factor involved in chromosome segregation or cytokinesis.
Two alternatively spliced transcript variants encoding the same protein have been noted for CDCA4.
Two pseudogenes have also been identified on chromosome 1.
Applications:Suitable for use in Immunohistochemistry (paraffin), Immunofluorescence, ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human CdcA4. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa90-170 of human CdcA4 at the Internal region.
