The protein encoded by CDC42 (cell division cycle 42) is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression.
This protein is highly similar to saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant.
The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein.
This protein could regulate actin polymerization through its direct binding to neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex.
Alternative splicing of CDC42 results in multiple transcript variants.
Pseudogenes of CDC42 have been identified on chromosomes 3, 4, 5, 7, 8 and 20.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human CDC42. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa80-160 from human CDC42.
