Cyclin dependent kinase 1 encoded by CDK1 is a member of the Ser/Thr protein kinase family.
Cyclin dependent kinase 1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle.
Mitotic cyclins stably associate with this protein and function as regulatory subunits.
The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle.
The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
Alternatively spliced transcript variants encoding different isoforms have been found for this CDK1.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Cdc2 (phospho Thr161). Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from human Cdc2 around the phosphorylation site of T161.
