CD2AP (CD2 associated protein) encodes a scaffolding molecule that regulates the actin cytoskeleton.
The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains.
The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells.
It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis.
Haploinsufficiency of CD2AP is implicated in susceptibility to glomerular disease.
CD2AP is a protein coding gene.
Diseases associated with CD2AP include glomerulosclerosis, focal segmental, 3 and sporadic idiopathic steroid-resistant nephrotic syndrome with focal segmental hyalinosis.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human CD2AP. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa320-400 from human CD2AP.
