CD160 is an 27kD glycoprotein which was initially identified with the monoclonal antibody BY55.
Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity.
The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181aa with a single Ig-like domain weakly homologous to KIR2DL4 molecule.
CD160 is expressed at the cell surface as a tightly disulfide-linked multimer.
RNA blot analysis revealed CD160 mRNAs of 1.5kb and 1.6kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine.
Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells.
In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes.
CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules.
Applications:Suitable for use in Western Blot, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human CD160.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa40-120 of human CD160.
