84-5427-73　［取扱停止］CD110 30ul　534215

特徴

• In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages.
• In 1992 the human homologue, named, c-mpl, was cloned.
• Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily.
• Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation.
• The ligand for c-mpl, thrombopoietin, was cloned in 1994.
• Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation.
• The protein encoded by the c-mpl gene, CD110, is a 635aa transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs.
• TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation.
• Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.