Catenin beta 1 encoded by CTNNB1 is part of a complex of proteins that constitute adherens junctions (AJs).
AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells.
Catenin beta 1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete.
Finally, catenin beta 1 binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon.
Mutations in CTNNB1 are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer.
Three transcript variants encoding the same protein have been found for CTNNB1.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Catenin-beta (phospho Ser552). Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human Catenin-beta around the phosphorylation site of S552.
