Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules.
CASP12 is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines.
In rodents, the homolog of CASP12 mediates apoptosis in response to endoplasmic reticulum stress.
However, in humans CASP12 contains a polymorphism for the presence or absence of a premature stop codon.
The majority of human individuals have the premature stop codon and produce a truncated non-functional protein.
The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis.
Several alternatively spliced transcript variants have been noted for CASP12.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Caspase 12.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the Internal region of human Caspase12.
