Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein.
It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm.
The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits.
The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation.
The protein encoded by this gene represents the alpha subunit.
While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11.
Three transcript variants encoding two different proteins have been found for this gene.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Casein Kinase IIalpha (phospho Tyr255). Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human Casein Kinase Ialpha around the phosphorylation site of Y255.
