Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer.
Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex.
After translocation, RanGTP binds importin-beta and displaces importin-alpha.
Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind.
The protein encoded by CSE1L binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1.
In addition, the encoded protein (chromosome segregation 1 like) may play a role both in apoptosis and in cell proliferation.
Alternatively spliced transcript variants have been found for CSE1L.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunoprecipitation (lysate): 2-5ug/mgImmunofluorescence: 1:200-1:1000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human CAS.
Isotype：IgG
Calc Applications Abbrev：E IF IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa1-80 of human CAS at N-terminal.
