CAGE (cancer-associated gene protein), also known as DEAD box protien 53 (DDX53) or DEAD box protein CAGE, belongs to the DEAD box helicase family.
It contains one helicase ATP-binding domain, one helicase C-terminal domain and one KH domain.
CAGE localizes to the nucleus and, in normal adult tissues, is exclusively expressed in testis but it has also been found in a wide variety of cancer tissues and cell lines.
Overexpression of CAGE leads to the activation of FAK, ERK and p38 MAPK along with a reduction in reactive oxygen species (ROS).
It is also responsible for inducing catalase activity and therefore enhancing cell motility.
This suggests that CAGE may enhance the migration of cancer cells.
In addition, hypomethylation of the CAGE promoter region is associated with tumor progression and may serve as a valuable marker in cancer diagnosis.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human CAGE-1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa680-760 of human CAGE-1 at C-terminal.
