Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA.
CAF-I is composed of three protein subunits, p50, p60, and p150.
The protein encoded by CHAF1B (chromatin assembly factor 1 subunit B) corresponds to the p60 subunit and is required for chromatin assembly after replication.
The encoded protein is differentially phosphorylated in a cell cycle-dependent manner.
In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm.
This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human CAF-1 p60. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa40-120 from the Internal region of human CAF-1 p60.
