The protein encoded by CA8 (carbonic anhydrase 8) was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes.
However, the gene product lacks carbonic anhydrase activity (i.e.
The reversible hydration of carbon dioxide).
The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family.
The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form.
Mutations in CA8 are associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3).
Polymorphisms in CA8 are associated with osteoporosis, and overexpression of CA8 in osteosarcoma cells suggests an oncogenic role.
Alternative splicing results in multiple transcript variants.
Applications:Suitable for use in Immunohistochemistry, ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human CA VIII. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the Internal region of human CA VIII.
