RAPGEF1 encodes a human guanine nucleotide exchange factor.
It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases.
This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation.
Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: paraffinELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human C3G (phospho Tyr504).
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human C3G around the phosphorylation site of Y504.
