This intronless gene (CEBPB) encodes a transcription factor (CCAAT/enhancer binding protein beta) that contains a basic leucine zipper (bZIP) domain.
CCAAT/enhancer binding protein beta functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma.
Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes.
The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of human C/EBP beta (phospho Thr235). Species Crossreactivity: mouse and rat
Isotype:IgG
Calc Applications Abbrev:E IF IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide derived from human C/EBP beta around the phosphorylation site of T235