This intronless gene (CEBPA) encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes.
The encoded protein (CCAAT/enhancer binding protein alpha) functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma.
Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis.
Mutation of CEBPA is associated with acute myeloid leukemia.
The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths.
Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human C/EBP alpha. Species Crossreactivity: mouse, rat, monkey
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to human C/EBP alpha around the non-phosphorylation site of T226.
