BCL2 associated agonist of cell death encoded by BAD is a member of the BCL-2 family.
BCL-2 family members are known to be regulators of programmed cell death.
BCL2 associated agonist of cell death positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity.
Proapoptotic activity of BCL2 associated agonist of cell death is regulated through its phosphorylation.
Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of BCL2 associated agonist of cell death.
Alternative splicing of BAD results in two transcript variants which encode the same isoform.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Bad (phospho Ser136). Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from human Bad around the phosphorylation site of S136.
