AZI1, also known as 5-azacytidine-induced protein 1, is a 1083aa protein that may play a role in spermatogenesis.
AZI1 is most highly expressed in spinal cord, followed by testis, ovary, amygdala, cerebellum and thalamus.
Low expression is present in other adult and fetal tissues and specific adult brain regions.
AZl1 gene transcription begins in pachytene spermatocytes and expression of the gene is induced in cultivated fibroblasts on treatment with 5-azacytidine, which is known to lead to the demethylation of genomic DNA.
The AZI1 gene is conserved in canine, cow, mouse, rat, chicken, and zebrafish, and exists as two alternatively spliced isoforms.
AZI1 contains one IQ domain, and the gene that encodes it maps to human chromosome 17q25.3.
Chromosome 17 makes up over 2.5% of the human genome with about 81 million bases encoding over 1200 genes.
Alexander disease, Birt-Hogg-Dube syndrome and Canavan disease are also associated with chromosome 17.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human AZI1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa280-360 of human AZI1.
