ATP5G3 (ATP synthase, H+ transporting, mitochondrial Fo complex subunit C3) encodes a subunit of mitochondrial ATP synthase.
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation.
ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel.
The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3.
The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8).
ATP5G3 is one of three genes that encode subunit c of the proton channel.
Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein.
Alternatively spliced transcript variants encoding different proteins have been identified.
Applications:Suitable for use in Immunohistochemistry, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human ATP5G3. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa10-90 from human ATP5G3 at N-terminal.
