84-5412-06　［取扱停止］ATOH8 100ul　533370

特徴

• Transcription factor that binds a palindromic (canonical) core consensus DNA sequence 5-CANNTG- 3 known as an E-box element, possibly as a heterodimer with other bHLH proteins.
• Regulates endothelial cell proliferation, migration and tube-like structures formation.
• Modulates endothelial cell differentiation through NOS3.
• May be implicated in specification and differentiation of neuronal cell lineages in the brain.
• May participate in kidney development and may be involved in podocyte differentiation.
• During early embryonic development is involved in tissue-specific differentiation processes that are dependent on class II bHLH factors and namely modulates the differentiation program initiated by the pro-endocrine factor NEUROG3.
• During myogenesis, may play a role during the transition of myoblasts from the proliferative phase to the differentiation phase.
• Positively regulates HAMP transcription in two ways, firstly by acting directly on the HAMP promoter via E-boxes binding and indirectly through increased phosphorylation of SMAD protein complex.
• Repress NEUROG3-dependent gene activation in a gene-specific manner through at least two mechanisms; requires only either the sequestering of a general partner such as TCF3 through heterodimerization, either also requires binding of the bHLH domain to DNA via a basic motif.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.