JUN is the putative transforming gene of avian sarcoma virus 17.
It encodes a protein (Jun proto-oncogene, AP-1 transcription factor subunit) which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression.
JUN is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunoprecipitation (lysate): 2-5 ug/mgELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:30ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of human AP-1. Species Crossreactivity: mouse, rat
Isotype:IgG
Calc Applications Abbrev:E IHC IP WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide corresponding to human AP-1 around the non-phosphorylation site of Y170.