Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network.
Mutations of ankyrin genes lead to severe genetic diseases, such as fatal cardiac arrhythmias and hereditary spherocytosis.
ANKRD30A (ankyrin repeat domain 30A), also known as serologically defined breast cancer antigen NY-BR-1, is a 1397aa coiled-coil protein.
Containing six ANK repeats and thirty-seven exons, ANKRD30A is a distant homolog of the POTE family.
Initiated by either Dnmt1 or Met-57, ANKRD30A is highly expressed in breast and testis, and faintly in placenta.
Present in 80% of breast cancers, but not in other types of tumors, ANKRD30A is a differentiation antigen and a potential target for antibody‐based therapies in breast cancers.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human ANKRD30A.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa1010-1090 of human ANKRD30A at C-terminal.
