Actin proteins are major components of the eukaryotic cytoskeleton.
At least six vertebrate actin isoforms have been identified.
The cytoplasmic beta- and gamma-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility.
The alpha-cardiac and alpha-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively.
The smooth muscle alpha-actin and gamma-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively.
The alpha-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin.
These actin isoforms regulate the contractile potential of muscle cells .
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Immunohistochemistry (paraffin): 1:50-1:300ELISA: 1:10,000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of alpha-SMA at ~42kD. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa171-220 of alpha-SMA
