The protein encoded by ALDOA, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.
Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development.
Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle.
Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue.
Aldolase A deficiency has been associated with myopathy and hemolytic anemia.
Alternative splicing and alternative promoter usage results in multiple transcript variants.
Related pseudogenes have been identified on chromosomes 3 and 10.
Applications:Suitable for use in Western Blot, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:200-1:1000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Aldolase A. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa1-80 of human Aldolase A at N-terminal.
