The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell.
AKAP12 (A-kinase anchoring protein 12) encodes a member of the AKAP family.
The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells.
It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction.
This protein and RII PKA colocalize at the cell periphery.
This protein is a cell growth-related protein.
Antibodies to this protein can be produced by patients with myasthenia gravis.
Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Applications:Suitable for use in Immunohistochemistry, Immunofluorescence, ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human AKAP 250.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa270-350 at the Internal region of human AKAP 250.
