Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton.
Three types of actins, alpha, beta and gamma, have been identified in vertebrates.
Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus.
The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
ACTG2 encodes actin gamma 2; a smooth muscle actin found in enteric tissues.
Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Based on similarity to peptide cleavage of related actins, the mature protein of ACTG2 is formed by removal of two N-terminal peptides.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Actin alpha 3. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa1-80 of human Actin alpha 3 at N-terminal.
