84-5396-21　［取扱停止］3 pK 100ul　532533

特徴

• MAPKAPK3 encodes a member of the Ser/Thr protein kinase family (mitogen-activated protein kinase-activated protein kinase 3).
• This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase.
• MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals.
• This kinase was shown to be activated by growth inducers and stress stimulation of cells.
• In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses.
• This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.
• Alternate splicing results in multiple transcript variants that encode the same protein.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.