HLA-DRB1 belongs to the HLA class II beta chain paralogs.
The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane.
It plays a central role in the immune system by presenting peptides derived from extracellular proteins.
Class II molecules are expressed in antigen presenting cells.
The beta chain is approximately 26-28kD.
It is encoded by 6 exons.
Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail.
Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities.
Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5.
DRB1 is present in all individuals.
Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5.
There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human 2B1F.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human 2B1F protein.
