This chimeric rabbit antibody was made using the variable domain sequences of mouse 4G2.
It binds to flavivirus group antigen, protein E and can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis, anti-Yellow Fever Virus or anti-Zika Virus antibody (Aubry et al., 2016) to identify cells infected with these flaviviridae.
It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation (Summers, 1989).
The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail (Crill & Chang, 2004).
Applications:Suitable for use in Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Neutralization and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: paraffinWestern Blot: non-reducing conditionsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% Proclin 300.
Specificity：Recognizes flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.
