Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum.
Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN).
Plays a role in the regulation of MF axon pathway choice.
Promotes MF migration towards ipsilaterally-located cerebellar territories.
May have a role in shear flow mechanotransduction in osteocytes.
Retains nuclear GLI1 and GLI3 in the cytoplasm.
Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3' (By similarity).
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence/Immunocytochemistry (IF-IC): 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human ZIC1/2/3. Species Crossreactivity: Mouse
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human ZIC1/2/3.
