84-5331-23　ZCCHC11 100ul　529781

特徴

• Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:25480299).
• Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets.
• Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c.
• Uridylated miRNAs are not processed by Dicer and undergo degradation.
• Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cell pluripotency (By similarity).
• Does not bind RNA directly, but recruited to RNA targets by RNA-binding protein LIN28A.
• Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript.
• This abrogates the silencing of IL6 transcript, hence promoting cytokine expression (By similarity).
• May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP.
• Does not play a role in replication-dependent histone mRNA degradation.
• Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.