84-5325-69　UMOD 100ul　529494

特徴

• This gene encodes uromodulin, the most abundant protein in normal urine.
• Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle.
• Uromodulin may act as a constitutive inhibitor of calcium crystallization in renal fluids.
• Excretion of uromodulin in urine may provide defense against urinary tract infections caused by uropathogenic bacteria.
• Defects in this gene are associated with the autosomal dominant renal disorders medullary cystic kidney disease-2 (MCKD2) and familial juvenile hyperuricemic nephropathy (FJHN).
• These disorders are characterized by juvenile onset of hyperuricemia, gout, and progressive renal failure.
• While several transcript variants may exist for this gene, the full-length natures of only two have been described to date.
• These two represent the major variants of this gene and encode the same isoform.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.