UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase.
It may participate in methylation-dependent transcriptional regulation.
UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression.
It is important for G1/S transition and may be involved in DNA repair and chromosomal stability.
UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry, ELISA, Immunoprecipitation, CHIP.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:20-1:50Immunoprecipitation: 1:20-1:50ChIP: 1:20-1:50ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human UHRF1. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：ChIP E IC IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human UHRF1. Species sequence homology: Porcine (100%), Zebrafish (100%), Bovine (100%), Equine (92%), Sheep (100%), Canine (100%), Chicken (100%), Xenopus (92%)
