84-5325-33　UGDH 100ul　529476

特徴

• UDP-GlcDH (also called UDP-glucose 6-dehydrogenase, UGDH or UDPGDH) is a member of the UDP-glucose/GDP- mannose dehydrogenase family.
• UDP-GlcDH converts UDP- glucose to UDP-glucuronic acid, which is a crucial component in the biosynthesis of the glycosaminoglycans, hyaluronan, heparan sulfate and chondroitin sulfate.
• Found as common components of the extracellular matrix, these glycosaminoglycans are significant in signal transduction, cell migration, cancer growth and cancer metastasis.
• UDP- glucuronic acid (UDP-GlcA) is needed in the liver for the excretion of toxic compounds.
• UDP-GlcDH is a ubiquitously expressed protein most abundant in the liver.
• The protein structure of UDP-GlcDH was first analyzed in cow liver and found to be a homohexamer.
• This structure is well conserved between species and phyla with an overall 97% sequence identity shared between different species of mammals.
• Research indicates that UDP-GlcDH expression is upregulated by TGF∫ and downregulated by hypoxia.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.