84-5325-25　UCP3 100ul　529472

特徴

• The uncoupling protein UCP1 (formerly designated UCP) is an integral membrane protein unique to brown adipose tissue mitochondria.
• UCP1 forms a dimer that acts as a proton channel, which can uncouple oxidative phosphorylation by dissipating the electrochemical potential across the inner mitochondrial membrane.
• This process induces heat production in brown adipose tissue and is involved in regulation of body temperature and glucose metabolism.
• UCP2 is a structurally related protein that also uncouples mitochondrial respiration.
• It is more widely expressed in human and mouse tissues, including white adipose tissue and muscle, than is UCP1.
• UCP2 is thought to play a role in body weight regulation.
• An additional UCP family member, UCP3, is highly muscle specific and is possibly involved in the uncoupling of oxidative phosphorylation in skeletal muscle.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.